The co-crystal structure of Cbl-b and a small-molecule inhibitor reveals the mechanism of Cbl-b inhibition

Cbl-b is a RING-type E3 ubiquitin ligase that is expressed in several immune cell lineages, where it negatively regulates the activity of immune cells. Cbl-b has specifically been identified as an attractive target for cancer immunotherapy due to its role in promoting an immunosuppressive tumor environment. A Cbl-b inhibitor, Nx-1607, is currently in phase I clinical trials for advanced solid tumor malignancies. Using a suite of biophysical and cellular assays, we confirm potent binding of C7683 (an analogue of Nx-1607) to the full-length Cbl-b and its N-terminal fragment containing the TKBD-LHR-RING domains. To further elucidate its mechanism of inhibition, we determined the co-crystal structure of Cbl-b with C7683, revealing the compound’s interaction with both the TKBD and LHR, but not the RING domain. Here, we provide structural insights into a novel mechanism of Cbl-b inhibition by a small-molecule inhibitor that locks the protein in an inactive conformation by acting as an intramolecular glue.


P
ost-translational modification by ubiquitination, the covalent conjugation of ubiquitin (Ub) to protein substrates, is an essential mechanism that regulates protein functions by altering their stability, cellular localization, activity, and proteinprotein interactions in diverse biological processes.Ub modification requires consecutive actions of three classes of enzymes termed E1, E2 and E3 1,2 .Ubiquitin activating enzyme E1 activates and transfers Ub to a ubiquitin conjugating enzyme E2, resulting in covalent attachment of Ub to the E2 catalytic cysteine.Subsequently, an E3 ligase recruits both a protein substrate and E2-Ub thus facilitating Ub transfer from the E2 to the lysine residues of the protein substrate 3 .E3s thus confer substrate specificity during ubiquitination, and defects in their function are associated with many disease pathologies including cancers, metabolic disorders, and neurodegenerative diseases 4,5 .
The human Casitas B lymphoma-b (Cbl-b) protein is a member of the monomeric Cbl family of RING-type E3 ubiquitin ligases that are known to downregulate non-receptor and receptor protein tyrosine kinase (PTK) signaling by ubiquitinating and thereby targeting these kinases for proteasomal or lysosomal degradation 6,7 .The Cbl family of E3 ligases share a highly conserved N-terminal region that contains the structural elements required for ubiquitin ligase activity 8,9 , including a substrate recognition tyrosine kinasebinding domain (TKBD), a short linker helix region (LHR) and a RING finger domain.The TKB domain is composed of three subdomains, a 4-helix bundle (4H), a calcium binding domain with an EF-hand fold (EF hand), and a variant Src homology region 2 (SH2) domain, all of which form a unique phosphotyrosinerecognition module 10 in which phosphorylated PTKs, such as ZAP-70 and Syk, are recruited for ubiquitination 11,12 .The RING finger domain directly associates with Ub conjugated E2 proteins and mediates target ubiquitination 13 .
The LHR comprises an N-terminal loop (linker loop 1), a helix, and a C-terminal loop (linker loop 2) and contains a strictly conserved tyrosine residue (Y363 in Cbl-b) within the linker helix (LH) that is essential for regulating the Cbl proteins E3 ligase activity.Phosphorylation of this critical tyrosine activates the Cbl proteins, as its mutation to phenylalanine abolishes the E3 ligase activity 14 .In the unphosphorylated state, Cbl proteins are autoinhibited through the interaction of TKBD with the conserved tyrosine and several other residues within the linker helix, resulting in the RING finger domain being restricted to the side of the TKBD, opposite to the substrate binding site 8,15 .In this inactive state, the RING finger domain can either be in a closed conformation unable to bind E2s, or in a substrate bindinginduced open catalytically competent conformation that can interact with E2 proteins 8,13,16 .Upon phosphorylation, the LHR is released from the TKBD, which allows the protein to undergo conformational changes that activate the protein.The activation process involves (i) unmasking of the RING finger domain from the TKBD, (ii) flipping of the RING domain around 180 degrees so it's positioned adjacent to the substrate-binding site on TKBD, (iii) pTyr-LHR forming interactions with the RING domain that stabilize E2-Ub during Ub transfer, and (iv) the phosphorylated tyrosine interacting directly with Ub thus generating a structural element adjacent to the RING finger domain that enhances its catalytic efficacy 3,15,16 .
In addition to the highly conserved N-terminus, Cbl-b and its closely related homolog c-Cbl contain a highly variable C-terminal extension that plays adapter-like functions by mediating interactions with several protein families 8 .The C-terminus consists of a proline rich region (PRR) that mediates binding to SH3 domain-containing proteins, a tyrosine rich region that can recruit SH2 domain-containing proteins following phosphorylation of the tyrosines 17 , and an ubiquitin-associated domain (UBA) that is essential for homodimerization and heterodimerization between Cbl-b and c-Cbl 18 and for binding ubiquitinated proteins, by Cbl-b only 18,19 .
Cbl-b is expressed in several immune cell lineages where it modulates both innate and adaptive immune responses, effectively playing a key role in the host defense mechanisms against pathogens and anti-tumor immunity 11 .Specifically, Cbl-b is a negative regulator of signaling pathways involving immune cell receptors, and it is particularly well-documented to play a central role in inhibiting effector T cell activation 20 .T cell activation requires both T cell receptor stimulation and CD28 receptor costimulation, which potentiates Cbl-b ubiquitination and degradation.In the absence of CD28 co-stimulation, Cbl-b inhibits T cell transcriptional activity through ubiquitination and degradation of various signaling transducers (as reviewed by Tang et al. 11 ), hence promoting immune suppression in both adaptive and innate immunity 21 .This inhibitory effect is significant in cancer development and progression as it promotes an immunosuppressive tumor environment, making Cbl-b an attractive therapeutic target for cancer immunotherapy, as well as other human immune-related disorders including infections and autoimmune diseases 11,22 .
Using a high throughput screening, Sharp et al. identified and developed a triazole based compound series targeting the E3 ligase activity of Cbl-b, including Nx-1607, which is currently in clinical trials as a cancer immunotherapeutic for the treatment of solid tumor malignancies (clinical trials.govID: NCT05107674) 23,24 .These compounds were shown to inhibit the interaction between the N-terminal E3 ligase fragment (TKBD-LHR-RING) of Cbl-b and UbcH5B-Ub (E2-Ub) complex in the presence of the Src kinase substrate (Patent number: WO2019148005), thus leading to the stimulation of immune cells in vitro and significant inhibition of tumors in vivo.However, the structural understanding of how these compounds interact with Cbl-b is lacking and hence their mechanism of action is not well characterized.Here, we report the co-crystal structure of the N-terminal E3 ligase fragment of Cbl-b in complex with a compound related to Nx-1607, which reveals that the Nx-1607 compound series bind to the TKBD-LHR unit of Cbl-b, locking the protein in an inactive conformation.

Results
The TKB domain and the linker helix region are required for C7683 binding to Cbl-b.Sharp et al (2022) reported a series of triazole compounds targeting the E3 ligase activity of Cbl-b for cancer immunotherapy 23,24 .These compounds were shown to inhibit the interaction of Cbl-b with a ubiquitin-charged UbcH5B (E2-Ub) complex using a TR-FRET assay (Patent number: WO2019148005).In the absence of any structural information or other molecular mechanistic data, the mode of inhibition of Cbl-b by these compounds remains poorly understood.Therefore, we aimed to investigate the mechanism of action of a compound from this class, using C7683 as an exemplar, and to simultaneously develop a toolkit for characterizing Cbl-b inhibitors.To accomplish this, we cloned, expressed and purified three variants of the Cbl-b protein (see Supplementary Fig. 1 for the Cbl-b protein domain schematic and an annotated Cbl structure), including the full-length protein, the TKBD-LHR-RING fragment (residues 38-427), and the RING domain alone (residues 351-426).
We first evaluated the binding activity of C7683, a representative triazole compound with isoindolin-1-one core structure that belongs to the same class as Nx-1607 reported in the patent application (WO2020264398), by differential scanning fluorimetry (DSF) 25 .Both the full-length Cbl-b and the TKBD-LHR-RING fragment exhibited significant stabilization by C7683 at 3 μM concentration (Fig. 1a, b), displaying ΔT m values of 10 ± 0.4 and 12 ± 0.2 °C, respectively.The observed stabilization by C7683 was concentration dependent (Fig. 1c, d), indicating a stoichiometric binding.This assay could be utilized in screening for novel chemical series targeting the N-terminal domain of Cbl-b.
Having confirmed the binding of C7683 to the full-length and the TKBD-LHR-RING Cbl-b proteins, we then focused on identifying the domain responsible for binding to C7683.As similar compounds were shown to inhibit the interaction of Cbl-b with UbcH5B-Ub complex in a TR-FRET based assay (Patent number, WO2019148005), we speculated that they may be binding to the RING domain of Cbl-b, which is an E2 binding module.
We first evaluated the binding of C7683 to the full-length, the TKBD-LHR-RING and the RING domain Cbl-b proteins using an orthogonal SPR assay.C7683 binds to both TKBD-LHR-RING and full-length proteins (Fig. 2a-d), yielding K D values of 8 ± 4 nM and 12 ± 6 nM, respectively, but did not show any significant binding to the RING domain-only protein (Supplementary Fig. 2a, b) suggesting that the TKBD/LHR regions are necessary for binding.
To confirm this further, we selected a fluorescein-labeled compound (C7102, Fig. 2) related to C7683 and reported in a patent application (Compound 46, WO2020210508) to develop an FP-based displacement assay 26 .In this assay, C7683 displaced binding of C7102 to both TKBD-LHR-RING and full-length proteins in a concentration dependent manner (Fig. 2e, f) but did not show any significant binding to the RING domain alone (Supplementary Fig. 2c), confirming that the RING domain is not involved in C7683 binding.The DSF ΔT m , the SPR K D and the FP K disp values are shown in Supplementary Table 1.
C7683 stabilizes Cbl-b protein in the cells.To further investigate the C7683 interaction with Cbl-b protein in cells, we developed a HiBIT target engagement cellular thermal shift assay (CETSA), which measures shifts in protein thermal stability following compound treatment.In this assay, HiBIT-tagged fulllength Cbl-b was expressed in HEK293T cells and cells were treated with increasing C7683 concentrations, exposed to a temperature gradient, and lysed in a buffer containing LgBIT protein.HiBIT complementation with LgBIT results in formation of the functional NanoLUC luciferase enzyme, which produces bioluminescence signal following substrate addition, and is dependent on the amount of soluble HiBIT protein at each temperature.C7683 showed significant dose-dependent stabilization of Cbl-b protein in cells (Fig. 3).Significant thermal stabilization was observed at concentration as low as 1 nM, with thermal shifts of >15 °C observed at 1 μM to 10 μM compound concentration, consistent with our biophysical thermal shifts assay results (Fig. 1).

C7683 binds at the interface of the TKBD and LHR of Cbl-b.
To further characterize the C7683 interaction with Cbl-b and understand its mechanism of action, we co-crystallized the N-terminal E3 ligase fragment of Cbl-b containing the TKBD, LHR and RING domains (residue range 38-427) in complex with C7683.Initial co-crystallization trials with a TKBD-LHR-RING protein containing a Tyrosine 360 mutation to Phenylalanine (similar to PDB ID: 3ZNI 3 ) generated poorly diffracting crystals in our hands.To improve the crystal packing and obtain better diffracting crystals, we introduced three-point mutations on the surface of the Cbl-b TKB domain by mutating residues K51, K55, R325 to alanines (the mutant protein referred to here as 3mCblb), which yielded crystals diffracting to 1.8 Å resolution.These mutations are located far from the substrate binding site as well as the regulatory regions involved in Cbl-b activation.The co-crystal structure of 3mCbl-b-C7683 (PDB: 8GCY) was determined in P2 1 2 1 2 1 space group symmetry, with one copy of the Cbl-b in the crystal asymmetric unit bound to C7683.Table 1 summarizes the crystallographic data collection, refinement, and validation statistics.
A well-resolved electron density map was observed for the entire C7683 molecule (Supplementary Fig. 3) and Cbl-b protein, except for residues 339-353 in the flexible linker helix region (LHR).C7683 was nestled in an interface between the 3 TKBD subdomains and the LHR (Fig. 4a, b), consistent with our biophysical studies, which showed that the compound binding does not involve the RING domain.C7683 binds in an extended   conformation, making extensive interactions with the residues of all three TKBD subdomains, the LHR and coordinating a nearby water molecule (HOH10) (Fig. 4c, d).The methyl-substituted piperidine ring is nestled in a pocket between the 4H and the SH2 subdomains, where it makes hydrophobic and van der Waals contacts with C289, T265, P71 and P72, in addition to a hydrogen bonding interaction between its nitrogen atom (N31) and the side chain oxygen (OE1) of E268 in the SH2 domain.The Isoindolin-1-one ring is also located between 4H and SH2 subdomains, making hydrophobic interactions with atoms from T144, K145, L148 in the 4H domain and L264 in the SH2, and hydrogen bonds to the backbone nitrogen of F263 in the SH2 domain through its oxygen atom.The phenyl ring is nestled between the side chains of L222 and M366 in the SH2 domain and the LHR respectively, while the methyl-substituted cyclobutane ring makes hydrophobic contacts with the side chains of T219, and L287 in the Ca 2+ binding EF hand and the SH2 domains, respectively.The triazole ring interacts primarily with the SH2 domain and the LHR; it makes two hydrogen bonds to the hydroxyl group of Y260 and a nearby water molecule (HOH10).The triazole ring is also involved in π-π stacking interaction with the side chain ring of the critical tyrosine (Y363) in the LHR, which plays a crucial role in regulating Cbl-b's ligase activity, as well as hydrophobic interactions with S218 and G367 (Fig. 4c, d).
C7683 locks Cbl-b in an inactive conformation.Comparison of the 3mCbl-b-C7683 structure with those of active and inactive conformations of Cbl-b and the closely-related c-Cbl 3,13,16 reveal that the compound bound Cbl-b adopts an inactive conformation, with the LHR being docked between the TKBD subdomains, and the RING finger domain being positioned on the side of the TKBD, opposite to the substrate binding site 3,8,15,16 (Fig. 5 & Supplementary Fig. 4).
As described above, C7683 interacts with the LHR through residues including M366 and the conserved Y363 (Fig. 4b).The activation of Cbl-b is dependent on the phosphorylation of Y363, which releases the LHR from interaction with the TBKD, allowing the protein to undergo large conformational changes (Fig. 5 & Supplementary Fig. 4) that promote effective ligase activity.These changes include exposure of the E2-binding surface of the RING domain to facilitate efficient interaction with the E2 ligase, and bringing the E2-bound RING domain into close proximity to the TKBD substrate binding region of Cbl-b 8,16 (Fig. 5 & Supplementary Fig. 4).The compound binding at the interface between the TKBD and LHR, as well as its specific interaction with the Y363 in the flexible LHR, locks the protein in an inactive conformation that prevents phosphorylation of Y363, which is crucial for Cbl-b activation.
A comparison of the compound-bound 3mCbl-b-C7683 structure with that of unbound inactive Cbl-b (PDB ID: 3VGO) 15 shows that the TKBD/LHR interface is not altered upon compound binding (Fig. 6a).In substrate-bound and activated forms of Cbl proteins (PDB ID: 2Y1N, PDB ID: 1FBV and PDB ID: 3ZNI) 3,13,16 , the SH2 domain loop containing F263 is displaced by approximately 4 Å following both substrate binding and tyrosine phosphorylation 16 (Fig. 6b).C7683 makes numerous contacts with this loop and fills the gap created in the TKBD domain of inactive Cbl-b conformation that otherwise would be occupied by SH2 domain loop following substrate binding and Y363 phosphorylation (Fig. 6).Thus, C7683 would not only prevent Y363 phosphylation for Cbl-b activation, but it would also block the conformational changes resulting from substrate binding to the TKBD domain of Cbl-b.

Discussion
In this study, we have structurally and biochemically characterized the mechanism of Cbl-b inhibition by C7683, a new class of E3 ligase inhibitor related to Nx-1607, which is currently in clinical trials for treatment of advanced solid tumor malignancies.We show that C7683 binds Cbl-b with high affinity and significantly stabilizes the protein both in vitro and in cellular assays.Importantly, we show that C7683 interacts with the TKBD and LHR of Cbl-b, thereby acting as an intramolecular glue by locking the protein in an inactive conformation (Fig. 7).
This presents a novel mechanism for the inhibition of ubiquitination activity of an E3 ligase by locking the nonphosphorylated form of the protein in a closed conformation, and without directly binding to the E2-binding RING domain.Other E3 ligases, mainly the HECT domain E3 ligases such as WWP1, WWP2, NEDD4 27-29 also adopt similar closed  autoinhibited state, in which its multiple WW domains sequester HECT using a multi-lock mechanism and phosphorylation of linker or cancer associated mutation relieve autoinhibition.As compounds targeting E2-binding HECT domain or substrate binding domains of these type of E3 ligases are limited, similar to this novel mechanism, HECT type E3 ligases could be inhibited by compounds stabilizing the inactive closed conformation.
In the case of Cbl-b, C7683 induced a large thermal shift as observed in vitro by DSF assay (Fig. 1) and in cells by CETSA assay (Fig. 3).These simple thermal shift assays could be used to identify novel inhibitors of Cbl-b as well as for other E3 ligases such as HECT E3 ligases acting via the same mechanism.
Here, we have developed target enabling resources for the human Cbl-b protein, an attractive target for cancer immunotherapy and other human immune disorders.Specifically, we have generated structural data and developed biophysical and cellular assays to support future drug development efforts to selectively inhibit Cbl-b and other Cbl E3 ligases involved in human diseases.

Methods
Protein expression and purification for biophysical binding characterization.Biophysical experiments were carried out using three Cbl-b protein variants: the full-length (1-982aa), the N-terminal E3 ligase fragment consisting of TKBD-LHR-RING (38-427aa) and the RING finger domain (351-426aa).The fulllength Cbl-b gene was cloned into an in-house Baculovirus expression vector, pFBD-BirA, a derivative of pFastBac Dual vector from Invitrogen, containing an N-terminal AviTag, a C-terminal hexa-His tag and co-expression of BirA.Biotinylated Cbl-b protein was expressed in Sf9 insect cells using biotinsupplemented media, following a protocol described by Hutchinson and Seitova 30 .The N-terminal E3 ligase fragment and the   16 , (ii), the c-Cbl in complex with a ZAP-70 substrate peptide (c-Cbl-S) (PDB ID: 2Y1N) 16 , (iii) the c-Cbl in complex with ZAP-70 peptide and UbcH7 E2 ligase (c-Cbl-S-E2) (PDB ID: 1FBV) 13 , and (iv) the pTyr363 Cbl-b in complex with ZAP-70 peptide, UbcH5B E2 ligase, and ubiquitin (pCbl-b-S-E2-Ub) (PDB ID: 3ZNI) 3 .structures (both inactive).The F263-bearing loop, as well as the amino acid sidechains in the vicinity of the compound overlap well in both structures, indicating that binding of the compound does not induce changes in the binding site.b A superposition of C7683-bound, active and inactive Cbl structures.The C7683-bound structure (green), Cbl-b apo structure (blue, PDB ID: 3VGO) 15 and c-Cbl apo structure (red, PDB ID: 2Y1M) 16 have the F263-bearing loop in the same conformation, which represents an inactive closed Cbl conformation.The c-Cbl protein in complex with a ZAP-70 substrate peptide structure (cyan, PDB ID: 2Y1N) 16 , the c-Cbl protein in complex with ZAP-70 peptide and UbcH7 E2 ligase structure (gray, PDB ID: 1FBV) 13 , and the pTyr363 Cbl-l structure in complex with ZAP-70 peptide, UbcH5B E2 ligase, and ubiquitin (brown, PDB ID: 3ZNI) 3 have the F263-bearing loop that is crucial for compound binding shifted by ~4 Å as indicated by the black arrow -which represents an open, inactive substrate-bound or active (phosphorylated) conformation.
RING domain genes were cloned into a pET28-derived in-house E. coli expression vector, p28BIOH-LIC, containing an N-terminal AviTag and a C-terminal hexa-His tag.The two proteins were co-expressed with BirA contained in a pBirAcm vector (Avidity LLC) in E. coli BL21 cells using biotinsupplemented media.All the proteins were purified using Nickel affinity chromatography, followed by size exclusion chromatography using a HiLoad TM 26/60 Superdex TM 75 gel filtration column (GE Healthcare).
Compound synthesis.The C7683 and fluorescein-labeled C7102 compounds were synthesized as reported in patent applications (WO2020210508, WO2020264398).
Thermal shift assay by Differential Scanning Fluorimetry (DSF).DSF 25 experiments were performed using a Roche LightCycler 480 II in sealed Axygen 384-well plates (PCR-384-LC480).Reactions (20 μL) contained 0.1 mg/mL TKBD-LHR-RING and full-length Cbl-b, in 30 mM Hepes pH 7.5, 150 mM NaCl, 0.5 mM TCEP, and SYPRO Orange (5000× SYPRO Orange stock in DMSO, Invitrogen).C7683 was tested at a serially diluted range from 0.01 to 100 µM with a final concentration of 2% DMSO.Thermal denaturation was monitored from 20 to 95 °C at a rate of 4 °C increase per minute, and data points were collected at 0.5 °C intervals.

Analysis of binding by Surface plasmon resonance (SPR).
In vitro binding analyses by SPR were carried out using a Biacore T200 (GE Health Sciences Inc.) instrument at 20 °C.20 mM Hepes, pH 7.5, 100 mM NaCl, 0.5 mM TCEP, 0.01% Triton X-100 was used for all the experiments.Biotinylated TKBD-LHR-RING, full-length, and RING domain Cbl-b proteins were immobilized yielding approximately 4000, 9000, and 2000 response units (RU), respectively, on a flow cell of a Streptavidincoated (SA) sensor chip (GE healthcare) according to manufacturer's directions while another flow cell was left blank for reference subtraction.The C7683 compound was diluted in the same buffer to yield a 2% final DMSO solution and was serially diluted 3-fold.The compound was then flowed over the sensor chip using a single cycle Kinetics mode at 50 μL/min.Contact time (i.e., association phase) was 50 s and disassociation time was 180 s.Buffer with 2% DMSO was used for blank injections; and buffers containing 1-3% DMSO were used for solvent correction injections.Binding constants were acquired from the double referenced (i.e., reference subtraction and blank injection subtraction) single cycle data using Biacore T200 Evaluation Software 3.1.Cellular target engagement.We performed a Cbl-b cellular thermal shift assay (CETSA) using NanoLuc split luciferase technology (Promega).HEK293T cells were plated in 6-well plates (4 e5 cells/ml) and 4 h later transfected with 0.2 µg of N-terminally HiBIT-tagged full-length Cbl-b and 1.8 µg of empty plasmid using X-tremeGene XP transfection reagent, following manufacturer's instructions.Next day cells were trypsinized and resuspended in OptiMEM (no phenol red) at a density of 2e5/ml.After compound or DMSO addition (the same DMSO concentration in each sample) cells were transferred to 96-well pcr plates (50 µl/well), incubated for 1 h at 37 °C and heated at indicated temperatures in thermocycler for 3 min.After 3 min at RT, 50 µl of LgBIT solution (200 nM LgBIT, 2% NP-40, prot.inhibitors in OptiMEM no phenol red) was added and incubated at RT for 10 min.Next, 25 µl of NanoGlo substrate (8 µl/ml) was added, mixed gently and 20 µl was transferred to 384 white plates in quadruplicates and bioluminescence signal was read.
Cbl-b gene cloning, protein expression and purification for protein crystallography.The N-terminal TKBD-LHR-RING fragment (residues 38-427) of human Cbl-b gene (UniProtKB Q13191) was cloned into an in-house pET28-derived E. coli expression vector called pET28-MHL, yielding a construct with an N-terminal His 6 -tag followed by a TEV cleavage site.Crystal engineering was then carried out to reduce the surface entropy by mutating residues K51, K55 and R325 into alanines to promote crystallization.The triple mutant Cbl-b fragment 3mCbl-b protein was expressed overnight at 16 °C in E. coli BL21 (DE3) pRARE2 cells.
Protein purification was performed by immobilized Nickel ion affinity chromatography, followed by size exclusion chromatography using a HiLoad TM 16/60 Superdex TM 75 gel filtration column (GE Healthcare).The protein was then subjected to TEV protease digestion overnight at 4 °C to remove polyhistidine purification tag.The protein was further purified by anion exchange chromatography using a SOURCE TM 15 Q column (MILLIPORE SIGMA) and eluted in the final protein buffer containing 20 mM Tris-HCl pH 7.5, 165 mM NaCl, 2.5% Glycerol and 2 mM TCEP.Protein fractions containing pure Cbl-b protein as confirmed by SDS-PAGE were pooled and concentrated using 10 kDa cutoff spin columns (Millipore), and the final protein concentration determined using the NanoDrop UV-Vis spectrophotometer (Thermo Scientific), with the protein extinction coefficient of 62340 M-1cm-1 computed from the amino acid sequence using Expasy ProtParam (https://web.expasy.org/protparam/).
Co-crystallization of Cbl-b in complex with C7683.To generate Cbl-b-C7683 co-crystals, purified 3mCbl-b protein at 10.7 mg/ml (0.237 mM) concentration was mixed with 3 times molar excess of C7683 (0.712 mM) and incubated at room temperature for 15 min prior to setting crystallization trays.Crystallization was carried out using the sitting drop vapor-diffusion method by mixing the protein-C7683 complex with an equal volume of the reservoir solution over 100 μL reservoir.Crystals were observed within 72 h at 18 °C in a precipitant solution containing 0.1 M Imidazole, 0.1 M MES monohydrate pH 6.5, 0.09 M Sodium nitrate, 0.09 M Sodium phosphate dibasic, 0.09 M Ammonium sulfate, 12.5% v/v MPD; 12.5% PEG1000; 12.5% w/v PEG 3350.
Diffraction data collection, structure determination and refinement.Crystals in the crystallization mother liquor that already contained cryoprotectant (MPD) were directly cryocooled in liquid nitrogen.Diffraction data were collected at 0.9791 nM wavelength on beamline 24-ID-E at the Advanced Photon Source in Argonne National Laboratory.The diffraction data were processed with HKL3000 31 and the structure was solved by molecular replacement in Phaser 32 using the apo Cbl-b crystal structure (PDB ID: 3VGO) 15 as a starting model.The model was refined by alternating cycles of manual rebuilding in Coot 33 and refinement with Refmac 34 within the CCP4 crystallography suite 35 .The structure was validated using the Molprobity server 36 and determined to have 100% residues in the allowed and 97.04% residues in the favored regions of the Ramachandran plot, with no outliers.The molecular graphic images were rendered using the PyMOL Molecular Graphics System, Schrodinger, LLC, and the protein-compound interactions 2D depiction was generated with ICM-Pro (v.3.8-2c, MolSoft CA, USA)).Molecular mechanism illustrations were made with Biorender.com.

Statistics and reproducibility.
For in vitro binding data, all the experiments were conducted in triplicate (n = 3).For each replicate, samples were processed independently to ensure reproducibility and minimize bias.The presented values represent the averages from n = 3 technical replicates, and the statistical analyses conducted using GraphPad Prism Version 9.5.1 represents the standard deviation resulting from the analyzed replicates.For cellular data, the experiment was performed in 4 technical replicates.The presented values represent non-linear fits of mean intensities ±SD generated from the four replicates using GraphPad Prism Version 9.0.2.The experiment was repeated one more time with similar results.
Reporting summary.Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.

Fig. 1
Fig. 1 C7683 potently stabilizes both full-length and TKBD-LHR-RING Cbl-b proteins in DSF assays.The chemical structure of C7683 is presented.C7683 stabilized (a) TKBD-LHR-RING and (b) full-length Cbl-b at 3 μM with ΔT m values of 10 ± 0.4 and 12 ± 0.2 °C, respectively.The stabilization effect of C7683 was concentration dependent (c, d) for both constructs at concentrations ranging from 0.01 to 100 µM.All experiments were performed in triplicates (n = 3).

Fig. 2
Fig. 2 C7683 shows potent binding to both full-length and TKBD-LHR-RING Cbl-b proteins in SPR and FP-based displacement assays.The chemical structure of the fluorescein-labeled probe (C7102) is presented.a-d Serially diluted C7683 was flowed over separately immobilized (a, c) TKBD-LHR-RING, and (b, d) full-length proteins.The representative sensorgrams for both sets of experiments are shown (solid red lines) with the kinetic fit (black dots), and the steady-state response (black circles) with the steady state 1:1 binding model fitting (red dashed line).C7683 was tested for competing with the fluorescein-labeled probe (C7102) for binding to (e) TKBD-LHR-RING and (f) full-length proteins at concentrations ranging from 0.001 to 20 µM.All experiments were performed in triplicate (n = 3) and the calculated K D and K disp values are presented in Supplementary Table1.
Fig. 2 C7683 shows potent binding to both full-length and TKBD-LHR-RING Cbl-b proteins in SPR and FP-based displacement assays.The chemical structure of the fluorescein-labeled probe (C7102) is presented.a-d Serially diluted C7683 was flowed over separately immobilized (a, c) TKBD-LHR-RING, and (b, d) full-length proteins.The representative sensorgrams for both sets of experiments are shown (solid red lines) with the kinetic fit (black dots), and the steady-state response (black circles) with the steady state 1:1 binding model fitting (red dashed line).C7683 was tested for competing with the fluorescein-labeled probe (C7102) for binding to (e) TKBD-LHR-RING and (f) full-length proteins at concentrations ranging from 0.001 to 20 µM.All experiments were performed in triplicate (n = 3) and the calculated K D and K disp values are presented in Supplementary Table1.
Fig. 2 C7683 shows potent binding to both full-length and TKBD-LHR-RING Cbl-b proteins in SPR and FP-based displacement assays.The chemical structure of the fluorescein-labeled probe (C7102) is presented.a-d Serially diluted C7683 was flowed over separately immobilized (a, c) TKBD-LHR-RING, and (b, d) full-length proteins.The representative sensorgrams for both sets of experiments are shown (solid red lines) with the kinetic fit (black dots), and the steady-state response (black circles) with the steady state 1:1 binding model fitting (red dashed line).C7683 was tested for competing with the fluorescein-labeled probe (C7102) for binding to (e) TKBD-LHR-RING and (f) full-length proteins at concentrations ranging from 0.001 to 20 µM.All experiments were performed in triplicate (n = 3) and the calculated K D and K disp values are presented in Supplementary Table1.

Fig. 3
Fig. 3 C7683 potently stabilizes Cbl-b in cells -HiBIT CETSA assay.As described in the material and methods, the amount of unaggregated HiBITtagged Cbl-b was quantified.C7683 significantly stabilized the Flag-Hb-Cbl-b protein in a dose-dependent manner compared to DMSO control.The results are MEAN+/−SD, n = 4.

Fig. 4 X
Fig. 4 X-ray crystal structure of Cbl-b N-terminal fragment bound to C7683. a Domain organization of the crystallized Cbl-b N-terminal fragment.b Side and top views of the 3mCbl-b-C7683 showing the compound nestled in an interface between the three TKBD subdomains and the linker helix region (LHR) of Cbl-b.The TKBD subdomains are shown as cartoon representation and colored according to the domain organization in panel a, and the compound is shown as magenta spheres.c A close-up view of the C7683 binding site.C7683 forms a network of interactions with a water molecule (red sphere), the three TKBD subdomains and the LHR.Hydrogen bond interactions are shown in black dashes.The compound is shown as magenta sticks and key interacting residues are rendered as sticks.d A 2D depiction of the protein-compound interactions.Blue shading represents hydrogen bonding residues and interactions, green shading hydrophobic interactions and gray shading other van der Waals contacts.

Fig. 6
Fig. 6 Comparison of the compound binding pocket of 3mCbl-b-C7683 with that of active and inactive Cbl structures.a An overlay of the 3mCbl-b-C7683 (green, C7683 magenta sticks) and the apo Cbl-b (blue, PDB ID: 3VGO) 15 structures (both inactive).The F263-bearing loop, as well as the amino acid sidechains in the vicinity of the compound overlap well in both structures, indicating that binding of the compound does not induce changes in the binding site.b A superposition of C7683-bound, active and inactive Cbl structures.The C7683-bound structure (green), Cbl-b apo structure (blue, PDB ID: 3VGO)15 and c-Cbl apo structure (red, PDB ID: 2Y1M)16 have the F263-bearing loop in the same conformation, which represents an inactive closed Cbl conformation.The c-Cbl protein in complex with a ZAP-70 substrate peptide structure (cyan, PDB ID: 2Y1N)16 , the c-Cbl protein in complex with ZAP-70 peptide and UbcH7 E2 ligase structure (gray, PDB ID: 1FBV)13 , and the pTyr363 Cbl-l structure in complex with ZAP-70 peptide, UbcH5B E2 ligase, and ubiquitin (brown, PDB ID: 3ZNI)3 have the F263-bearing loop that is crucial for compound binding shifted by ~4 Å as indicated by the black arrow -which represents an open, inactive substrate-bound or active (phosphorylated) conformation.
Fluorescence polarization (FP)-based displacement assay.The FP-based displacement experiments26 were performed in 384well polypropylene small volume black microplates (Cat.# 784209, Greiner) in a total volume of 20 µL per well at room temperature (i.e., 23 °C).C7683 compound was added to the reaction mixture containing 850 nM TKBD-LHR-RING, fulllength and RING domain Cbl-b proteins, and 40 nM of the fluorescein-labeled probe (C7102) in buffer (20 mM Hepes, pH 7.4, 150 mM NaCl, 0.01% Triton X-100, 0.5 mM TCEP), and were incubated for 30 min at room temperature.Final DMSO concentration was 2%.The resulting FP signals were measured using a BioTek Synergy 4 (BioTek, Winooski, VT) at the excitation and emission wavelengths of 485 nm and 528 nm, respectively.The obtained FP values were blank subtracted and are shown as the percentage of control.All the experiments were carried out in triplicate (n = 3) and the presented values are the average of replicates ± standard deviation.FP data were visualized using GraphPad Prism software 8.0 (La Jolla, CA).

Fig. 7 A
Fig. 7 A summary of the mechanism of Cbl-b inhibition by C7683.C7683 binds at the interface of TKBD sub-domains (4H, EF hand and SH2-like) and the LHR, acting as an intramolecular glue that locks the Cbl-b protein in an inactive conformation.Inhibition of Cbl-b subsequently activates immune cells, in line with the ongoing clinical applications of C7683-related compounds as anti-cancer immunotherapeutics.Figure created with BioRender.com.

Table 1
Data collection and refinement statistics (molecular replacement).
a A single crystal was used to solve the structure.b Values in parentheses are for highest-resolution shell.